Obtaining “ghosts” of red blood cells with a set ion concentration as models for studying Na+, K+ - ATPase

The article considers the production of red blood cell “ghosts” and their application as a model for studying Na+, K+ - ATPase. Various variants liposomes are used test objects ion transport, but best biological membranes transport ATPase pumps «ghosts» cells. activity Na+/K+ is determined mainly in suspension “ghosts”. This due to need remove interfering compound – hemoglobin obtain that necessary accurate measurement enzyme. In this scientific work, studies were conducted determine concentrations Na+ ions cells cows calves various ages by atomic absorption emission spectroscopy. values these “Ghosts” with set concentration sodium potassium obtained method J. T. Dodge, modification Zhumadilov Zh. Sh. Gening P. our modification. Studies showed full compliance calculated values.